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Foodborne Pathogens and Antimicrobial Resistance
Antibiotic Resistant Patterns of Bacterial Species Isolated from Vended Grilled Chicken Drumsticks
📊 1. Investigation Overview
This research evaluates the microbiological safety profiles of ready-to-eat grilled chicken drumsticks vended along major roadsides in Igbogene and Tombia. Due to open-air exposure, inadequate temperature controls, and varying handler hygiene, these products frequently act as vectors for multi-drug resistant bacterial pathogens, creating critical public health vulnerabilities.
⚙️ 2. Isolation & Biochemical Workflow
To accurately isolate and enumerate the contaminating microflora, a systematic serial dilution and selective culturing workflow was executed across specialized media variants:
💊 3. Antimicrobial Phenotypes & Resistance Transfer
Standardized in-vitro disk diffusion testing on Mueller-Hinton Agar (MHA) uncovered significant phenotypic resistance profiles against foundational classes of antibiotics including Beta-lactams and Fluoroquinolones.
Molecular tracking via plasmid DNA profiling verified that these multi-drug resistant strains carry heavy R-plasmids (exceeding 15kbp). These extrachromosomal structures dynamically facilitate rapid horizontal resistance gene transfer across varied microbial populations.
⚠️ Public Health Conclusions
The frequent tracking of multi-drug resistant isolates in ready-to-eat meats highlights a severe food safety gap. It is vital to mandate food safety protocols, proper holding temperatures, and strict vendor sanitization programs throughout Bayelsa State to protect the local population.
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